Over the last 15 years, HPLC has evolved into a powerful analytical technique, which is being applied to many different fields of study from research labs to quality control and regulatory facilities, in both government and private sectors. This course will cover origins, theoretical and instrumental aspects of operation as well as all requirements of today’s HPLC. It will include a review of chromatographic terminology and will cover topics like sample preparation, method development, detectors and some troubleshooting.